Micro BCA Protein Assay Kit
Technique: | Absorbance, Solution-based Detection |
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Assay Type: | Protein Quantitation Assay |
Product Size: | ۵۰۰ mL |
Detection Method: | Colorimetric |
For Use With (Equipment): | Microplate Reader, Spectrophotometer |
The Thermo Scientific Pierce Micro BCA Protein Assay Kit is a special 3-component version of our popular BCA Reagents, optimized to measure (A562nm) total protein concentration of dilute protein solutions (0.5 to 20 micrograms/mL).
Features of the Micro BCA Protein Assay Kit:
• Sensitive—accurately detect down to 0.5 µg/mL (2 µg/mL in microplate format)
• High linearity—linear working range for BSA equals 0.5 to 20 µg/mL
• Colorimetric—measure with a standard spectrophotometer or plate reader (562nm)
• Compatible—unaffected by typical concentrations of most ionic and nonionic detergents
• Convenient—microplate and cuvette protocols provided with the instructions
• Stable—kit stable at room temperature; prepared working reagent stable for to 24 hours
The Micro BCA Protein Assay Kit is a specialized version of the popular Pierce BCA Protein Assay for determining the protein concentration of dilute samples. Mixing together the three Micro BCA Reagents results in a working solution that is sufficiently concentrated to measure protein when mixed with an equal volume of sample. The result is an assay for accurately measuring 0.5 to 20 µg/mL protein solutions. The assay is exceptionally linear and exhibits very low levels of protein-to-protein variability.
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